Coding

Part:BBa_K209085:Design

Designed by: Ryan Quan   Group: iGEM09_UCSF   (2009-10-14)


PAKA-N-linker-PIPK1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 520
    Illegal PstI site found at 2482
    Illegal PstI site found at 2794
    Illegal PstI site found at 3088
    Illegal PstI site found at 3598
    Illegal PstI site found at 3698
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 520
    Illegal PstI site found at 2482
    Illegal PstI site found at 2794
    Illegal PstI site found at 3088
    Illegal PstI site found at 3598
    Illegal PstI site found at 3698
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 520
    Illegal BglII site found at 1819
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 520
    Illegal PstI site found at 2482
    Illegal PstI site found at 2794
    Illegal PstI site found at 3088
    Illegal PstI site found at 3598
    Illegal PstI site found at 3698
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 520
    Illegal PstI site found at 2482
    Illegal PstI site found at 2794
    Illegal PstI site found at 3088
    Illegal PstI site found at 3598
    Illegal PstI site found at 3698
    Illegal NgoMIV site found at 2677
    Illegal NgoMIV site found at 3398
    Illegal NgoMIV site found at 3566
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2697


Design Notes

Aar1 AB BC CD


Source

plasmid

References